63 dokumen ditemukan
(Menampilkan 1-20 | Halaman 1 dari 4)
Urutkan
#
Judul
Penulis
Tahun
Akses
Jurnal Institusi
Playground Facilities, Augmented Reality and K3L Training, As well as Making a Video Profile as an Effort to Develop the Potential of Educational Tourism in Kesiman Village
Dinamisia : Jurnal Pengabdian Kepada Masyarakat; Vol. 7 No. 3 (2023): Dinamisia: Jurnal Pengabdian Kepada Masyarakat; 817-826
Kesiman Village is located in the Trawas District of the Mojokerto Regency in East Java. Kesiman Village possesses unrealized natural potential and is currently attempting to become a tourist village. Therefore, the Community Empowerment Program Team...
Jurnal Institusi
Analisis Faktor-Faktor Penyebab Kendala Triple Constraint Proyek Konstruksi Akibat Pandemi Covid-19
Siklus : Jurnal Teknik Sipil; Vol. 7 No. 2 (2021); 160-174
Proyek konstruksi mempunyai sasaran (target) tertentu dengan batasan-batasan mutu pekerjaan, anggaran dan jadwal, yang dikenal sebagai triple constraint. Perbedaan keberhasilan proyek disebabkan karena tiap proyek mempunyai permasalahan/ken...
PubMed
Regulation of the protein kinase PKR by the vaccinia virus pseudosubstrate inhibitor K3L is dependent on residues conserved between the K3L protein and the PKR substrate eIF2alpha.
Mol Cell Biol
The mammalian double-stranded RNA-activated protein kinase PKR is a component of the cellular antiviral defense mechanism and phosphorylates Ser-51 on the alpha subunit of the translation factor eIF2 to inhibit protein synthesis. To identify the mole...
PubMed
Interaction of the interferon-induced PKR protein kinase with inhibitory proteins P58IPK and vaccinia virus K3L is mediated by unique domains: implications for kinase regulation.
Mol Cell Biol
Expression of the double-stranded RNA-activated protein kinase (PKR) is induced by interferons, with PKR activity playing a pivotal role in establishing the interferon-induced antiviral and antiproliferative states. PKR is directly regulated by physi...
PubMed
The vaccinia virus K3L gene product potentiates translation by inhibiting double-stranded-RNA-activated protein kinase and phosphorylation of the alpha subunit of eukaryotic initiation factor 2.
J Virol
Interferon resistance of vaccinia virus is mediated by specific inhibition of phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (eIF-2 alpha) by the double-stranded-RNA-activated (DAI) protein kinase. Vaccinia virus encodes a hom...
PubMed
Protein kinase PKR mutants resistant to the poxvirus pseudosubstrate K3L protein
Proc Natl Acad Sci U S A
As part of the mammalian cell innate immune response, the double-stranded RNA activated protein kinase PKR phosphorylates the translation initiation factor eIF2α to inhibit protein synthesis and thus block viral replication. Poxviruses including vac...
PubMed
Species‐specific inhibition of capripoxvirus replication by host antiviral protein kinase R
Ann N Y Acad Sci
The role of interferon (IFN)‐induced protein kinase R (PKR) in capripoxvirus (CaPV)‐infected cells remains unknown. In this study, we show that CaPV infection triggered PKR and eukaryotic translation initiation factor 2 alpha (eIF2α) protein pho...
PubMed
The E3L and K3L vaccinia virus gene products stimulate translation through inhibition of the double-stranded RNA-dependent protein kinase by different mechanisms.
J Virol
Vaccinia virus has evolved multiple mechanisms to counteract the interferon-induced antiviral host cell response. Recently, two vaccinia virus gene products were shown to interfere with the activity of the double-stranded RNA-dependent protein kinase...
PubMed
Protein kinase R reveals an evolutionary model for defeating viral mimicry
Nature
Distinguishing self from non-self is a fundamental biological challenge. Many pathogens exploit the challenge of self discrimination by employing mimicry to subvert key cellular processes including the cell cycle, apoptosis, and cytoskeletal dynamics...
PubMed
A highly efficient human cell-free translation system
RNA
Cell-free protein synthesis (CFPS) systems enable easy in vitro expression of proteins with many scientific, industrial, and therapeutic applications. Here we present an optimized, highly efficient human cell-free translation system that bypasses man...
PubMed
Poxviruses deploy genomic accordions to adapt rapidly against host antiviral defenses
Cell
In contrast to RNA viruses, double-stranded DNA viruses have low mutation rates, yet must still adapt rapidly in response to changing host defenses. To determine mechanisms of adaptation we subjected the model poxvirus vaccinia to serial propagation ...
PubMed
Ectromelia virus accumulates less double-stranded RNA compared to vaccinia virus in BS-C-1 cells
Virology
Most orthopoxviruses, including vaccinia virus (VACV), contain genes in the E3L and K3L families. The protein products of these genes have been shown to combat PKR, a host defense pathway. Interestingly, ectromelia virus (ECTV) contains an E3L orthol...
PubMed
Roles of Vaccinia Virus Genes E3L and K3L and Host Genes PKR and RNase L during Intratracheal Infection of C57BL/6 Mice
J Virol
The importance of the 2′-5′ oligoadenylate synthetase (OAS)/RNase L and double-stranded RNA (dsRNA)-dependent protein kinase (PKR) pathways in host interferon induction resulting from virus infection in response to dsRNA has been well documented....
PubMed
A highly efficient human cell-free translation system
bioRxiv
Cell-free protein synthesis (CFPS) systems enable easy in vitro expression of proteins with many scientific, industrial, and therapeutic applications. Here we present an optimized, highly efficient human cell-free translation system that bypasses man...
PubMed
Overcoming the eIF2α Brake in Human Cell-Derived Translation Systems
bioRxiv
Cell-free translation from human cells is a powerful platform for studying mammalian gene expression and building synthetic biology tools, but productivity is often curtailed by inhibitory phosphorylation of eIF2α on residue Ser52. Here we systemati...
PubMed
Ectopic Expression of Vaccinia Virus E3 and K3 Cannot Rescue Ectromelia Virus Replication in Rabbit RK13 Cells
PLoS One
As a group, poxviruses have been shown to infect a wide variety of animal species. However, there is individual variability in the range of species able to be productively infected. In this study, we observed that ectromelia virus (ECTV) does not rep...
PubMed
Application of poxvirus K3 ortholog as a positive selection marker for constructing recombinant vaccinia viruses with modified host range
MethodsX
Vaccinia virus is capable of replicating in a wide range of vertebrate animal cells. However, deletion of the two virus host range genes, E3L and K3L, would render replication of the virus abortive in all the cell types tested, except the cells with ...
PubMed
Single Immunization of a Vaccine Vectored by a Novel Recombinant Vaccinia Virus Affords Effective Protection Against Respiratory Syncytial Virus Infection in Cotton Rats
Front Immunol
Respiratory syncytial virus (RSV) is a leading cause of respiratory infections worldwide and disease management measures are hampered by the lack of a safe and effective vaccine against the infection. We constructed a novel recombinant RSV vaccine ca...
PubMed
A Single Amino Acid Dictates Protein Kinase R Susceptibility to Unrelated Viral Antagonists
PLoS Pathog
During millions of years of coevolution with their hosts, cytomegaloviruses (CMVs) have succeeded in adapting to overcome host-specific immune defenses, including the protein kinase R (PKR) pathway. Consequently, these adaptations may also contribute...
PubMed
Antagonism of Protein Kinase R by Large DNA Viruses
Pathogens
Decades of research on vaccinia virus (VACV) have provided a wealth of insights and tools that have proven to be invaluable in a broad range of studies of molecular virology and pathogenesis. Among the challenges that viruses face are intrinsic host ...