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Jurnal Institusi
Purification and Characterization of Amyloglucosidase Enzyme from the Thermophilic Endomycopsis fibuligera Using Sago Starch as a Substrate
Indonesian Journal of Chemistry; Vol 16, No 3 (2016); 302-307
An investigation on purification and characterization of amyloglucosidase enzyme from Endomycopsis fibuligera by fermentation of sago starch has been carried out. This enzyme is inductive and can be produced by fermenting sago starch in a medium cont...
Jurnal Institusi
Environmental literacy model in organic waste processing based on eco enzyme
Berkala Ilmu Perpustakaan dan Informasi; Vol 21 No 1 (2025): June; 125-141
Introduction. Community Reading Space or Taman bacaan masyarakat (TBM) can play a significant role in environmental literacy through literacy programs offered to residents. This study aims to examine the role and stages of environmental literacy in o...
Jurnal Institusi
Glucose Oxidase Immobilization on TMAH-Modified Bentonite
Indonesian Journal of Chemistry; Vol 15, No 1 (2015); 22-28
The influence of bentonite modification by tetramethyl ammonium hydroxide (TMAH) on its capability to immobilize glucose oxidase (GOX) was studied. Modification of bentonite was conducted by the adding of 0-5% (v/v) TMAH. The observed results show th...
Jurnal Institusi
Copper and Cadmium Toxicity to Marine Phytoplankton, Chaetoceros gracilis and Isochrysis sp.
Indonesian Journal of Chemistry; Vol 15, No 2 (2015); 172-178
In Copper (Cu) based antifouling (AF) paints Cu was largely used as booster biocide after organotin was banned. Cu is micronutrient which is important in photosynthesis process because Cu is an essential metal as component of enzyme and electron tran...
PubMed
angiotensin-converting enzyme 2 3.4.17.23
Class 3.4–6 Hydrolases, Lyases, Isomerases, Ligases
EC number 3.4.17.23 Recommended name angiotensin-converting enzyme 2 Synonyms ACE <4> [12] ACE 2 <10,12,13> [74] ACE-2 <2,3,4,9> [38,68] ACE-related carboxypeptidase <9> [3] ACE2 <1,2,3,4,5,6,9,10,11,12...
PubMed
GloEC: a hierarchical-aware global model for predicting enzyme function
Brief Bioinform
The annotation of enzyme function is a fundamental challenge in industrial biotechnology and pathologies. Numerous computational methods have been proposed to predict enzyme function by annotating enzyme labels with Enzyme Commission number. However,...
PubMed
Immunological Characterization of Maize Starch Branching Enzymes
Plant Physiol
Highly purified fractions of three starch branching enzymes from developing maize (Zea mays L.) endosperm were used to prepare antisera in rabbits. In double diffusion experiments, no immunoprecipitate was observed when branching enzyme IIa or IIb wa...
PubMed
Immunological Comparison of the Starch Branching Enzymes from Potato Tubers and Maize Kernels
Plant Physiol
Starch branching enzyme was purified from potato (Solanum tuberosum L.) tubers as a single species of 79 kilodaltons and specific antibodies were prepared against both the native enzyme and against the gel-purified, denatured enzyme. The activity of ...
PubMed
Enzyme synthesis in the regulation of hepatic `malic' enzyme activity
Biochem J
A homogeneous preparation of `malic' enzyme (EC 1.1.1.40) from livers of thyroxine-treated rats was used to prepare in rabbits an antiserum to the enzyme that reacts monospecifically with the `malic' enzyme in livers of rats in several phys...
PubMed
Exploring the competitive dynamic enzyme allocation scheme through enzyme cost minimization
ISME Commun
Enzyme allocation (or synthesis) is a crucial microbial trait that mediates soil biogeochemical cycles and their responses to climate change. However, few microbial ecological models address this trait, particularly concerning multiple enzyme functio...
PubMed
Presence of two DNA polymerases in Tetrahymena pyriformis.
Nucleic Acids Res
Two DNA polymerases were detected in Tetrahymena pyriformis, strain GL. One (enzyme I) was sensitive to N-ethylmaleimide, while the other (enzyme II) was insensitive. The molecular weight of the enzymes, as determined by glycerol gradient centrifugat...
PubMed
Harnessing Generative AI to Decode Enzyme Catalysis and Evolution for Enhanced Engineering
bioRxiv
Enzymes, as paramount protein catalysts, occupy a central role in fostering remarkable progress across numerous fields. However, the intricacy of sequence-function relationships continues to obscure our grasp of enzyme behaviors and curtails our capa...
PubMed
Isolation of a Pyrophosphoryl Form of Pyruvate, Phosphate Dikinase from Propionibacteria
Proc Natl Acad Sci U S A
Pyruvate, phosphate dikinase from Propionibacterium shermanii catalyzes the formation of P-enolpyruvate, AMP, and inorganic pyrophosphate from pyruvate, ATP, and orthophosphate; the mechanism involves three partial reactions and three forms of the en...
PubMed
Selective Elimination of the Exonuclease Activity of the Deoxyribonucleic Acid Polymerase from Escherichia coli B by Limited Proteolysis
Proc Natl Acad Sci U S A
Purification of DNA polymerase from E. coli B has in two cases each time led to the isolation of two separate polymerase activities, enzyme A and enzyme B. Enzyme A was in contrast to enzyme B almost completely devoid of exonuclease activity. Each of...
PubMed
Identification of the phosphocarrier protein enzyme IIIgut: essential component of the glucitol phosphotransferase system in Salmonella typhimurium.
J Bacteriol
The phosphoenolpyruvate-dependent phosphorylation of glucitol has been shown to require four distinct proteins in Salmonella typhimurium: two general energy-coupling proteins, enzyme I and HPr, and two glucitol-specific proteins, enzyme IIgut and enz...
PubMed
Starch Branching Enzymes from Maize
Plant Physiol
Spleen cells from mice immunized with starch branching enzymes were fused with cells from the mouse myeloma Sp2/0-AG14 cell line to form hybridomas. Those hybridomas producing antibodies against the branching enzyme were screened by the enzyme-linked...
PubMed
Regulation of enzyme levels in the blood. Influence of environmental and genetic factors on enzyme clearance.
Am J Pathol
Since its discovery, lactic dehydrogenase virus (LDV) has remained unique as a model of long-term enzyme elevation due to impairment of enzyme clearance. The present study shows that mice inoculated with silica develop an increase in plasma lactate d...
PubMed
Biochemical Characterization of an Acetylcholine-hydrolyzing Enzyme from Bean Seedlings
Plant Physiol
An acetylcholine hydrolyzing enzyme was prepared and purified (40 times) from dwarf bean hypocotyl hooks. The purity of the enzyme was proved by polyacrylamide gel electrophoresis. The molecular weight of the enzyme was determined to be 65,000 dalton...
PubMed
Research progress of multi-enzyme complexes based on the design of scaffold protein
Bioresour Bioprocess
Multi-enzyme complexes designed based on scaffold proteins are a current topic in molecular enzyme engineering. They have been gradually applied to increase the production of enzyme cascades, thereby achieving effective biosynthetic pathways. This pa...
PubMed
Interpretable out-of-distribution diagnostics for enzyme stability prediction
Brief Bioinform
AIM: Enzyme property predictors often degrade when deployed to new enzyme families whose sequence and label distributions differ from training data. Investigating how distribution shifts among enzyme families affect prediction error is crucial for bu...
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