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Jurnal Institusi
Efficacy of neem and basil leaf extracts in controlling whitefly (Bemisia Tabaci) infestations and enhancing yield in curly red chili peppers (Capsicum annuum L.)
Jurnal Ilmiah Pertanian; Vol. 21 No. 2 (2024): Jurnal Ilmiah Pertanian; 139-150
Whiteflies (Bemisia tabaci) are a major pest of curly red chili peppers, acting as vectors for gemini viruses that significantly reduce crop productivity. This study evaluated the efficacy of neem and basil leaf extracts at three different concentrat...
Jurnal Institusi
Pengaruh Kualitas Produk, Harga dan Promosi terhadap Keputusan Pembelian Kosmetik Lipstik Hanasui Di Pekanbaru (Studi Kasus Followers Instagram)
Jamalk: JURNAL MANAJEMEN LANCANG KUNING; Vol. 2 No. 5 (2024): JAMALK; 284-292
This inquiry endeavors to appraise corporate performance by interrogating the influence of product quality, price, and promotion on consumer purchasing determinations, specifically regarding Hanasui lipstick among Pekanbaru-based Instagram adherents....
Jurnal Institusi
Image Processing dan Artifical Neural Network Untuk Mengenali Nomor Induk Kependudukan Pada KTP Sebagai Pendukung Layanan Mandiri di Kantor Desa
Digital Zone: Jurnal Teknologi Informasi dan Komunikasi; Vol. 8 No. 2 (2017): Digital Zone: Jurnal Teknologi Informasi dan Komunikasi; 81-90
Abstrak- Pelayanan prima yang cepat dan tepat harus diberikan oleh pemerintah desa kepada masyarakat. Namun kesan layanan birokrasi pemerintahan berbelit-belit sudah menjadi pemikiran kebanyakan masyarakat. Hal ini didasari atas fakta yang masyarakat...
PubMed
Attaining the Promise of Geminivirus-Based Vectors in Plant Genome Editing
Viruses
Over the last 40 years, several studies have provided evidence demonstrating that viral vectors can result in effective gene targeting/insertions in a host’s genome. The traditional approaches of gene knock-down, -out, or -in involve an intensive t...
PubMed
Bioluminescence-Driven Optimization of Geminivirus-Based Vectors as Tools for Plant Biotechnology
ACS Synth Biol
Viral replicons are valuable tools in plant biotechnology, widely utilized to increase recombinant protein production. Their ability to amplify gene dosage in a trigger-dependent manner also opens doors to regulatory applications. This work focuses o...
PubMed
PubMed
Geminiviruses and Plant Hosts: A Closer Examination of the Molecular Arms Race
Viruses
Geminiviruses are plant-infecting viruses characterized by a single-stranded DNA (ssDNA) genome. Geminivirus-derived proteins are multifunctional and effective regulators in modulating the host cellular processes resulting in successful infection. Vi...
PubMed
Development of Modular Geminivirus‐Based Vectors for High Cargo Expression and Gene Targeting in Plants
Plant Biotechnol J
Viral vectors can be useful tools for expressing recombinant proteins as well as delivering gene‐editing machinery. Despite their utility, the development and subsequent optimisation of these tools is often a difficult and tedious process. Thus, al...
PubMed
Geminivirus-Derived Vectors as Tools for Functional Genomics
Front Microbiol
A persistent issue in the agricultural sector worldwide is the intensive damage caused to crops by the geminivirus family of viruses. The diverse types of viruses, rapid virus evolution rate, and broad host range make this group of viruses one of the...
PubMed
Genome editing in cells of apple cultivar ‘Fuji’ using geminivirus-derived replicons for transient expression of CRISPR/Cas9 components
Plant Biotechnol (Tokyo)
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system has been used for genome editing in various fruit trees, including apple (Malus × domestica). In previous studies, transfer DNA (T-D...
PubMed
Trans replication and high copy numbers of wheat dwarf virus vectors in maize cells.
Nucleic Acids Res
The replication of shuttle vectors derived from Wheat Dwarf Virus, a monopartite geminivirus, was studied in cultured maize endosperm cells, and in the Black Mexican Sweet (BMS) maize cell line. Using in vivo labeling and DNA methylation analysis, we...
PubMed
Co-Acquired Nanovirus and Geminivirus Exhibit a Contrasted Localization within Their Common Aphid Vector
Viruses
Single-stranded DNA (ssDNA) plant viruses belong to the families Geminiviridae and Nanoviridae. They are transmitted by Hemipteran insects in a circulative, mostly non-propagative, manner. While geminiviruses are transmitted by leafhoppers, treehoppe...
PubMed
Manipulation of the Plant Host by the Geminivirus AC2/C2 Protein, a Central Player in the Infection Cycle
Front Plant Sci
Geminiviruses are a significant group of emergent plant DNA viruses causing devastating diseases in food crops worldwide, including the Southern United States, Central America and the Caribbean. Crop failure due to geminivirus-related disease can be ...
PubMed
DNA Virus Vectors for Vaccine Production in Plants: Spotlight on Geminiviruses
Vaccines (Basel)
Plants represent a safe, efficacious and inexpensive production platform by which to provide vaccines and other therapeutic proteins to the world’s poor. Plant virus expression vector technology has rapidly become one of the most popular methods to...
PubMed
A Multipurpose Toolkit to Enable Advanced Genome Engineering in Plants
Plant Cell
We report a comprehensive toolkit that enables targeted, specific modification of monocot and dicot genomes using a variety of genome engineering approaches. Our reagents, based on transcription activator-like effector nucleases (TALENs) and the clus...
PubMed
A plant DNA virus replicates in the salivary glands of its insect vector via recruitment of host DNA synthesis machinery
Proc Natl Acad Sci U S A
Whereas most of the arthropod-borne animal viruses replicate in their vectors, this is less common for plant viruses. So far, only some plant RNA viruses have been demonstrated to replicate in insect vectors and plant hosts. How plant viruses evolved...
PubMed
CRISPR/Cas9 Targeted Editing of Genes Associated With Fungal Susceptibility in Vitis vinifera L. cv. Thompson Seedless Using Geminivirus-Derived Replicons
Front Plant Sci
The woody nature of grapevine (Vitis vinifera L.) has hindered the development of efficient gene editing strategies to improve this species. The lack of highly efficient gene transfer techniques, which, furthermore, are applied in multicellular expla...
PubMed
High-frequency, precise modification of the tomato genome
Genome Biol
BACKGROUND: The use of homologous recombination to precisely modify plant genomes has been challenging, due to the lack of efficient methods for delivering DNA repair templates to plant cells. Even with the advent of sequence-specific nucleases, whic...
PubMed
Transient Expression of Lumbrokinase (PI239) in Tobacco (Nicotiana tabacum) Using a Geminivirus-Based Single Replicon System Dissolves Fibrin and Blood Clots
Evid Based Complement Alternat Med
Lumbrokinases, a group of fibrinolytic enzymes extracted from earthworm, have been widely used to prevent and treat various cardiovascular diseases. They specifically target fibrin to effectively degrade thrombi without major side effects. Plant expr...
PubMed
Geminivirus-Mediated Gene Silencing from Cotton Leaf Crumple Virus Is Enhanced by Low Temperature in Cotton
Plant Physiol
A silencing vector for cotton (Gossypium hirsutum) was developed from the geminivirus Cotton leaf crumple virus (CLCrV). The CLCrV coat protein gene was replaced by up to 500 bp of DNA homologous to one of two endogenous genes, the magnesium chelatas...