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DOAJ Open Access
Engineering a broad-spectrum glucose oxidase via substrate channel and linker design for enhanced lignocellulose bioconversion
Synthetic and Systems Biotechnology, Vol 12, Iss , Pp 218-228 (2026) | ISSN: 2405-805X
Glucose oxidase (GOD) is a widely used enzyme in biotechnology, yet its narrow substrate specificity limits its application in complex bioconversion processes such as agricultural waste valorization. In this study, we employed synthetic biology and p...
Jurnal Institusi
Immobilization of Glucose Oxidase on Modified-Carbon-Paste-Electrodes for Microfuel Cell
Indonesian Journal of Chemistry; Vol 16, No 1 (2016); 92-97
Glucose oxidase (GOx) is being developed for many applications such as an implantable fuel cell, due to its attractive property of operating under physiological conditions. This study reports the functional immobilization of glucose oxidase onto poly...
Jurnal Institusi
Glucose Oxidase Immobilization on TMAH-Modified Bentonite
Indonesian Journal of Chemistry; Vol 15, No 1 (2015); 22-28
The influence of bentonite modification by tetramethyl ammonium hydroxide (TMAH) on its capability to immobilize glucose oxidase (GOX) was studied. Modification of bentonite was conducted by the adding of 0-5% (v/v) TMAH. The observed results show th...
Jurnal Institusi
Significance of Glucose Addition on Chitosan-Glycerophosphate Hydrogel Properties
Indonesian Journal of Chemistry; Vol 16, No 1 (2016); 65-71
Chitosan-glycerophosphate hydrogel can be used as dental scaffold due to its thermosensitivity, gelation performance at body temperature, suitable acidity for body condition, biocompatibility, and ability to provide good environment for cell prolifer...
Jurnal Institusi
Synthesis of Silver-Chitosan Nanocomposites Colloidal by Glucose as Reducing Agent
Indonesian Journal of Chemistry; Vol 15, No 1 (2015); 29-35
Silver-chitosan nanocomposites colloidal was successfully performed by chemical reduction method at room temperature using glucose as reducing agent, sodium hydroxide (NaOH) as accelerator reagent, silver nitrate (AgNO3) as metal precursor and chitos...
Jurnal Institusi
Detection of Hg2+ Metal Ions Using Silver Nanoparticles Stabilized by Gelatin and Tween-20
Indonesian Journal of Chemistry; Vol 15, No 1 (2015); 1-8
Silver nanoparticles were synthesized by reduction method using glucose as reducing agent for precursor AgNO3. This research was aimed at comparing the stability and performance of silver nanoparticles with stabilizer gelatin (Gelatin-AgNPs) and twee...
Jurnal Institusi
Polyaniline-Invertase-Gold Nanoparticles Modified Gold Electrode for Sucrose Detection
Indonesian Journal of Chemistry; Vol 15, No 3 (2015); 226-233
Sucrose sensor has been made by deposited the active materials on the surface of gold electrode. The active materials, i.e. polyaniline (PANI), invertase and gold nanoparticles, were deposited step by step. Aniline polymerization were conducted elect...
Jurnal Institusi
Purification and Characterization of Amyloglucosidase Enzyme from the Thermophilic Endomycopsis fibuligera Using Sago Starch as a Substrate
Indonesian Journal of Chemistry; Vol 16, No 3 (2016); 302-307
An investigation on purification and characterization of amyloglucosidase enzyme from Endomycopsis fibuligera by fermentation of sago starch has been carried out. This enzyme is inductive and can be produced by fermenting sago starch in a medium cont...
PubMed
In vitro analysis of the role of glucose oxidase from Talaromyces flavus in biocontrol of the plant pathogen Verticillium dahliae.
Appl Environ Microbiol
Culture filtrates from Talaromyces flavus grown on glucose contained high levels of glucose oxidase activity, while culture filtrates from T. flavus grown on xylan contained negligible glucose oxidase activity. Culture filtrates from T-flavus grown o...
PubMed
The Fluorescence Emission of the Apo-glucose Oxidase from Aspergillus niger as Probe to Estimate Glucose Concentrations
Biochem Biophys Res Commun
We developed a new method of glucose sensing using an inactive form of glucose oxidase from Aspergillus niger. Glucose oxidase was rendered inactive by removal of the FAD cofactor. The resulting apo-glucose oxidase still binds glucose as observed fro...
PubMed
Liquid-Phase Packaging of a Glucose Oxidase Solution with Parylene Direct Encapsulation and an Ultraviolet Curing Adhesive Cover for Glucose Sensors
Sensors (Basel)
We have developed a package for disposable glucose sensor chips using Parylene encapsulation of a glucose oxidase solution in the liquid phase and a cover structure made of an ultraviolet (UV) curable adhesive. Parylene was directly deposited onto a ...
PubMed
Insights into the Structures, Inhibitors, and Improvement Strategies of Glucose Oxidase
Int J Mol Sci
Glucose oxidase, which uses molecular oxygen as an electron acceptor to specifically catalyze the conversion of β-d-glucose to gluconic acid and hydrogen peroxide (H(2)O(2)), has been considered an important enzyme in increasing environmental sustai...
PubMed
Isolation and Characterization of a Catabolite Repression-Insensitive Mutant of a Methanol Yeast, Candida boidinii A5, Producing Alcohol Oxidase in Glucose-Containing Medium
Appl Environ Microbiol
Mutants exhibiting alcohol oxidase (EC 1.1.3.13) activity when grown on glucose in the presence of methanol were found among 2-deoxyglucose-resistant mutants derived from a methanol yeast, Candida boidinii A5. One of these mutants, strain ADU-15, sho...
PubMed
Evaluation of Different Disinfectants on the Performance of an On-Meter Dosed Amperometric Glucose-Oxidase-Based Glucose Meter
J Diabetes Sci Technol
BACKGROUND: Off-meter dosed photometric glucose-oxidase-based glucose meters have been reported to be susceptible to interference by hydrogen-peroxide-based disinfecting agents. The objective of this study was to determine if a single application of ...
PubMed
Glucose Oxidase Sensing: 3D Macroporous Zinc Compound/Silicone Hybrid Foams for Amperometric Sensing of Glucose Oxidase (Global Challenges 2/2019)
Glob Chall
In article number 1800049, Yingcheng Lin, Orhan Kizilkaya, Jian Xu, and co‐workers demonstrate a zinc compound/silicone hybrid foam that can be used for amperometric sensing of glucose oxidase. An increase in the concentration of the glucose oxidas...
PubMed
NADPH Oxidase versus Mitochondria-Derived ROS in Glucose-Induced Apoptosis of Pericytes in Early Diabetic Retinopathy
J Ophthalmol
Objectives. Using apocynin (inhibitor of NADPH oxidase), and Mitoquinol 10 nitrate (MitoQ; mitochondrial-targeted antioxidant), we addressed the importance of mitochondria versus NADPH oxidase-derived ROS in glucose-induced apoptosis of pericytes. Me...
PubMed
Following Glucose Oxidase Activity by Chemiluminescence and Chemiluminescence Resonance Energy Transfer (CRET) Processes Involving Enzyme-DNAzyme Conjugates
Sensors (Basel)
A hybrid consisting of glucose oxidase-functionalized with hemin/G-quadruplex units is used for the chemiluminescence detection of glucose. The glucose oxidase-mediated oxidation of glucose yields gluconic acid and H(2)O(2). The latter in the presenc...
PubMed
Determination of glucose oxidase activity by tyrosine fluorescence spectrophotometry
Heliyon
A novel Fe(2+)/Tyr/H(2)O(2) fluorescence reaction system has been established for the purpose of analyzing glucose oxidase activity. This system involves the catalysis of glucose oxidase on glucose to produce H(2)O(2), which in turn oxidizes tyrosine...
PubMed
Reconstitution of pyrroloquinoline quinone-dependent D-glucose oxidase respiratory chain of Escherichia coli with cytochrome o oxidase.
J Bacteriol
D-Glucose dehydrogenase is a pyrroloquinoline quinone-dependent primary dehydrogenase linked to the respiratory chain of a wide variety of bacteria. The enzyme exists in the membranes of Escherichia coli, mainly as an apoenzyme which can be activated...
PubMed
The dissociation of glucose oxidase by sodium n-dodecyl sulphate.
Biochem J
1. The enzymic activity of glucose oxidase was determined as a function of pH and sodium n-dodecyl sulphate (SDS) concentration. 2. Glucose oxidase is not deactivated by SDS at pH 6 even after prolonged incubation, but is deactivated at pH 4.3 and 3....
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