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Polyaniline-Invertase-Gold Nanoparticles Modified Gold Electrode for Sucrose Detection
Indonesian Journal of Chemistry; Vol 15, No 3 (2015); 226-233
Sucrose sensor has been made by deposited the active materials on the surface of gold electrode. The active materials, i.e. polyaniline (PANI), invertase and gold nanoparticles, were deposited step by step. Aniline polymerization were conducted elect...
PubMed
In silico analysis of the structural diversity and interactions between invertases and invertase inhibitors from potato (Solanum tuberosum L.)
3 Biotech
We performed sequence diversity, phylogenetic profiling, 3D structure modelling and in silico interactions between invertases (cell wall/apoplastic and vacuolar) and invertase inhibitors (cell wall/apoplastic and vacuolar) from potato. Cloning and se...
PubMed
Purification and characterization of neutral and alkaline invertase from carrot.
Plant Physiol
Neutral and alkaline invertase were identified in cells of a suspension culture of carrot (Daucus carota L.) and purified to electrophoretic homogeneity. Neutral invertase is an octamer with a molecular mass of 456 kD and subunits of 57 kD, whereas a...
PubMed
THE INFLUENCE OF PROTEINS ON THE REACTIVATION OF YEAST INVERTASE
J Gen Physiol
1. Acid-inactivated yeast invertase could not be regenerated in the presence of the proteolytic enzymes trypsin, pepsin, and chymotrypsin. 2. Certain foreign proteins of non-enzymatic nature partially inhibited the reactivation of acid-inactivated in...
PubMed
An Invertase Inactivator in Maize Endosperm and Factors Affecting Inactivation
Plant Physiol
A protein present in the developing endosperm of maize (Zea mays L.) causes a loss of invertase activity under certain conditions of incubation. This protein, designated an inactivator, inactivates invertase I of maize even in the presence of other p...
PubMed
Effect of Temperature on Invertase, Invertase Inhibitor, and Sugars in Potato Tubers
Plant Physiol
The accumulation of reducing sugars in potato tubers exposed to low temperatures occurs with concomitant formation of the enzyme invertase. During the initial period of cold treatment when reducing sugars increase rapidly, invertase formation proceed...
PubMed
Molecular Sieving by Neurospora Cell Walls During Secretion of Invertase Isozymes
J Bacteriol
Trevithick, John R. (University of Wisconsin Medical School, Madison), and Robert L. Metzenberg. Molecular sieving by Neurospora cell walls during secretion of invertase isozymes. J. Bacteriol. 92: 1010–1015. 1966.—The secretion of invertase by y...
PubMed
Invertase Activity in Normal and Mutant Maize Endosperms during Development
Plant Physiol
A bound invertase and two soluble invertases are found in the developing endosperm of maize (Zea mays L.). The two soluble invertases can be separated on diethylaminoethyl-cellulose and Sephadex columns and distinguished by their kinetic constants. O...
PubMed
Change in Invertase Activity of Sweet Potato in Response to Wounding and Purification and Properties of Its Invertases
Plant Physiol
When root tissue of sweet potato (Ipomoea batatas Lam.) was sliced, acid invertase activity, initially absent in freshly sliced tissue, appeared after a 3- to 6-hour lag phase, rapidly reached a maximum in 18 hours, and thereafter decreased. The incr...
PubMed
Regeneration of Invertase in Neurospora crassa
J Bacteriol
In Neurospora, invertase is predominately an extracellular enzyme, and acid phosphatase is partially external in location. Both extracellular invertase and acid phosphatase were rapidly and quantitatively inactivated by acid treatment (pH 1.3). When ...
PubMed
Promotion of Growth and Invertase Activity by Gibberellic Acid in Developing Avena Internodes
Plant Physiol
Gibberellic acid (GA(3)) induces invertase activity within 6 hours in Avena stem segments that are incubated in the dark at 23°. The maximum amount of promotion is about 5 times that of invertase activity in untreated segments. GA(3) causes signific...
PubMed
Enzymatic Activity Assay for Invertase in Synechocystis Cells
Bio Protoc
Invertase can catalyze the hydrolysis of sucrose, and is widely distributed in cells of cyanobacteria and plants. Being responsible for the first step for sucrose metabolism, invertase plays important physiological roles and its enzymatic activity is...
PubMed
Mutations affecting the signal sequence alter synthesis and secretion of yeast invertase.
Proc Natl Acad Sci U S A
Insertion mutations previously constructed within the proximal region of the yeast invertase signal sequence did not interfere with secretion or glycosylation of the enzyme. We now describe deletion mutations within the same signal sequence. Large de...
PubMed
Acid and Neutral Invertases in the Mesocarp of Developing Muskmelon (Cucumis melo L. cv Prince) Fruit
Plant Physiol
Acid and neutral invertases were found in the mesocarp of developing muskmelon (Cucumis melo L. cv Prince) fruit and the activities of these enzymes declined with maturation of the fruit, concomitantly with the accumulation of sucrose. Neutral invert...
PubMed
Genome-Wide Identification of the Invertase Gene Family in Populus
PLoS One
Invertase plays a crucial role in carbohydrate partitioning and plant development as it catalyses the irreversible hydrolysis of sucrose into glucose and fructose. The invertase family in plants is composed of two sub-families: acid invertases, which...
PubMed
Yeast invertase polymorphism is correlated with variable states of oligosaccharide chain phosphorylation.
Proc Natl Acad Sci U S A
Saccharomyces cerevisiae invertase (EC 3.2.1.26) isolated from wild-type strain X2180 can be resolved by isoelectric focusing into at least seven bands revealed by an activity stain. Most of this polymorphism is eliminated in mutants that are defecti...
PubMed
Production and localization of beta-fructosidase in asynchronous and synchronous chemostat cultures of yeasts.
Appl Environ Microbiol
In synchronized continuous cultures of Saccharomyces cerevisiae CBS 8066, the production of the extracellular invertase (EC 3.2.1.26) showed a cyclic behavior that coincided with the budding cycle. The invertase activity increased during bud developm...
PubMed
Genes Affecting the Regulation of SUC2 Gene Expression by Glucose Repression in SACCHAROMYCES CEREVISIAE
Genetics
Mutants of Saccharomyces cerevisiae with defects in sucrose or raffinose fermentation were isolated. In addition to mutations in the SUC2 structural gene for invertase, we recovered 18 recessive mutations that affected the regulation of invertase syn...
PubMed
Relationship of large and small invertases in Saccharomyces: mutant selectively deficient in small invertase.
J Bacteriol
A mutant strain of Saccharomyces cerevisiae (D10-ER1) has been isolated after a two-step mutagenesis of strain 4059-358D (SUC 1) using ethyl methane sulfonate. Cells of this new strain produced a level of total invertase equaling that of 4059 but con...
PubMed
The genetics of a putative social trait in natural populations of yeast
Mol Ecol
The sharing of secreted invertase by yeast cells is a well-established laboratory model for cooperation, but the only evidence that such cooperation occurs in nature is that the SUC loci, which encode invertase, vary in number and functionality. Geno...
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