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Jurnal Institusi
Purification and Characterization of Amyloglucosidase Enzyme from the Thermophilic Endomycopsis fibuligera Using Sago Starch as a Substrate
Indonesian Journal of Chemistry; Vol 16, No 3 (2016); 302-307
An investigation on purification and characterization of amyloglucosidase enzyme from Endomycopsis fibuligera by fermentation of sago starch has been carried out. This enzyme is inductive and can be produced by fermenting sago starch in a medium cont...
Jurnal Institusi
Evaluation of Lagergren Kinetics Equation by Using Novel Kinetics Expression of Sorption of Zn2+ onto Horse Dung Humic Acid (HD-HA)
Indonesian Journal of Chemistry; Vol 16, No 3 (2016); 338-346
Extraction and purification of humic acid from dry horse dung powder (HD-HA) was performed successfully and the purified HD-HA was then applied as sorbent to adsorb Zn2+. Extraction and purification were performed based on procedure of Stevenson (199...
Jurnal Institusi
Transformation of Eugenol and Safrole into Hydroxychavicol
Indonesian Journal of Chemistry; Vol 15, No 1 (2015); 77-85
Hydroxychavicol is found in betel leaf at low concentration and is reported to have antibacterial, anti-inflammatory, antioxidant, anticancer, and antimutagenic activities. This study aimed to synthesize hydroxychavicol from eugenol and safrole. Isol...
PubMed
血液净化在重症腺病毒肺炎患儿中的临床应用
Zhongguo Dang Dai Er Ke Za Zhi
OBJECTIVE: To study the role of blood purification in the treatment of severe adenovirus pneumonia. METHODS: A total of 57 children with severe adenovirus pneumonia who underwent mechanical ventilation from February to June, 2019, were enrolled. Acco...
PubMed
Surface processes during purification of InP quantum dots
Beilstein J Nanotechnol
Recently, a new simple and fast method for the synthesis of InP quantum dots by using phosphine as phosphorous precursor and myristic acid as surface stabilizer was reported. Purification after synthesis is necessary to obtain samples with good optic...
PubMed
A multi-column plate adapter provides an economical and versatile high-throughput protein purification system
Protein Expr Purif
Protein purification is essential in the study of protein structure and function, and the development of novel therapeutics. Many studies require purifying multiple proteins at once, increasing the demand for improved purification methods. We hypothe...
PubMed
Purification methods to reduce interference by dextran sodium sulfate with quantification of gene expression in intestinal tissue samples from a piglet model of colitis
J Anim Sci
Dextran sodium sulfate (DSS) is commonly used to induce intestinal (i.e., colonic) inflammation in a variety of animal models. However, DSS is known to cause interference when using quantitative-real time polymerase chain reaction (qRT-PCR) methods, ...
PubMed
Purification of anti-glycoconjugate monoclonal antibodies using newly developed porous zirconia particles
Sci Rep
Here, we describe porous zirconia particles (PZPs) optimized for the purification of immunoglobulins. PZPs, with a pore size of approximately 10 nm, were designed to specifically interact with antibodies via surface modification with a phosphate fun...
PubMed
Hofmeister series salts enhance purification of plasmid DNA by non-ionic detergents
Biotechnol Bioeng
Ion-exchange chromatography is the standard technique used for plasmid DNA purification, an essential molecular biology procedure. Non-ionic detergents (NIDs) have been used for plasmid DNA purification, but it is unclear whether Hofmeister series sa...
PubMed
Pancreatic Islet Purification from Large Mammals and Humans Using a COBE 2991 Cell Processor versus Large Plastic Bottles
J Clin Med
The islet purification step in clinical islet isolation is important for minimizing the risks associated with intraportal infusion. Continuous density gradient with a COBE 2991 cell processor is commonly used for clinical islet purification. However,...
PubMed
A High-Performance Polymer Composite Column for Coronavirus Nucleic Acid Purification
Res Sq
Here, we report the development of a novel polymer composite (PC) purification column and kit. The performance of the PC columns was compared to conventional silica gel (SG) columns for the purification of nucleic acids from coronaviruses, including ...
PubMed
A high-performance polymer composite column for coronavirus nucleic acid purification
Sci Rep
Here, we report the development of a novel polymer composite (PC) purification column and kit. The performance of the PC columns was compared to conventional silica gel (SG) columns for the purification of nucleic acids from coronaviruses, including ...
PubMed
Isolation of Bothrops jararaca Snake Antithrombin from the Supernatant of Fibrinogen Purification
J Biomol Tech
A novel method of antithrombin (AT) purification from Bothrops jararaca snake plasma was developed to obtain this protein using a waste supernatant from B. jararaca fibrinogen purification. The AT purification was achieved by affinity chromatography ...
PubMed
Preparation of Group I Introns for Biochemical Studies and Crystallization Assays by Native Affinity Purification
PLoS One
The study of functional RNAs of various sizes and structures requires efficient methods for their synthesis and purification. Here, 23 group I intron variants ranging in length from 246 to 341 nucleotides—some containing exons—were subjected to a...
PubMed
Analysis of the mediating effect of social status perception on professional identity and work engagement of blood purification nurses
Sci Rep
To explore the relationship between social status perception, professional identity, and work engagement among nurses involved in blood purification, and to provide a theoretical basis for improving the professional identity and work engagement of bl...
PubMed
Respiratory Benefits of Multisetting Air Purification in Children
JAMA Pediatr
IMPORTANCE: Particulate matter exposure has been linked to impaired respiratory health in children, but the respiratory benefits of air purification have not been fully elucidated. OBJECTIVES: To assess the respiratory health outcomes among children ...
PubMed
Magnetic Cytoskeleton Affinity (MiCA) Purification of Microtubule Motors conjugated to Quantum Dots
Bioconjug Chem
We develop Magnetic Cytoskeleton Affinity (MiCA) purification, which allows for rapid isolation of molecular motors conjugated to large multivalent quantum dots, in miniscule quantities, which is especially useful for single-molecule applications. Wh...
PubMed
Study on the ability of indoor plants to absorb and purify benzene pollution
Sci Rep
The ability of indoor plants to purify benzene pollution is the basic basis for the selection of plants for ecological remediation of indoor benzene pollution. In this study, the purification rate and the purification amount per unit leaf area of 13 ...
PubMed
Significance of Cartridges and Resins used in a Purification Column during (18)F-fluorodeoxyglucose Synthesis
Indian J Nucl Med
AIMS AND OBJECTIVE: The aims and objective of this study to share the 15 years of working experience regarding importance of cartridges and resins used in a Purification Column during 18F-FDG synthesis. MATERIALS AND METHODS: 18F- flourodeoxyglucose ...
PubMed
Pre‐folding purification procedures for inclusion body‐derived non‐tagged cationic recombinant proteins with multiple disulfide bonds for efficient refolding
Biotechnol Prog
The production of disulfide‐containing recombinant proteins often requires refolding of inclusion bodies before purification. A pre‐refolding purification step is crucial for effective refolding because impurities in the inclusion bodies interfer...
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