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Optimizing winged-bean meal through oven and autoclave heating as viable alternative for plant-based aquafeed
Jurnal Ilmiah Pertanian; Vol. 21 No. 2 (2024): Jurnal Ilmiah Pertanian; 84-92
Winged-bean meal can serve as a substitute for soybean meal in plant-based fish feed, despite its trypsin-inhibitor properties, which can be alleviated through heating processes. This study aimed to explore the potential of winged-bean meal subjected...
PubMed
Culex quinquefasciatus Late Trypsin Biosynthesis Is Translationally Regulated by Trypsin Modulating Oostatic Factor
Front Physiol
Trypsin is a serine protease that is synthesized by the gut epithelial cells of female mosquitoes; it is the enzyme that digests the blood meal. To study its molecular regulation, Culex quinquefasciatus late trypsin was purified by diethylaminoethyl ...
PubMed
Biochemical characterization of a native group III trypsin ZT from Atlantic cod (Gadus morhua)
Int J Biol Macromol
Atlantic cod trypsin ZT is biochemically characterized for the first time in this report in comparison to a group I trypsin (cod trypsin I). To our knowledge, trypsin ZT is the first thoroughly characterized group III trypsin. A more detailed underst...
PubMed
Functional modifications of α(2)-macroglobulin by primary amines. Kinetics of inactivation of α(2)-macroglobulin by methylamine, and formation of anomalous complexes with trypsin
Biochem J
The unique steric inhibition of endopeptidases by human α(2)M (α(2)-macroglobulin) and the inactivation of the latter by methylamine were examined in relation to each other. Progressive binding of trypsin by α(2)M was closely correlated with the l...
PubMed
Trypsin-Enhanced Infection with Porcine Epidemic Diarrhea Virus Is Determined by the S2 Subunit of the Spike Glycoprotein
J Virol
Porcine epidemic diarrhea virus (PEDV) is an enteric pathogen of importance to the swine industry, causing high mortality in neonatal piglets. Efficient PEDV infection usually relies on the presence of trypsin, yet the mechanism of trypsin dependency...
PubMed
ISOLATION FROM BEEF PANCREAS OF CRYSTALLINE TRYPSINOGEN, TRYPSIN, A TRYPSIN INHIBITOR, AND AN INHIBITOR-TRYPSIN COMPOUND
J Gen Physiol
Methods are described for the isolation and crystallization of trypsinogen, trypsin, a substance which inhibits trypsin, and an inhibitor-trypsin compound. Analyses and some of the properties of these compounds are given.
PubMed
Uncovering immobilized trypsin digestion features from large-scale proteome data generated by high-resolution mass spectrometry
J Chromatogr A
Immobilized trypsin produces very fast protein digestion, which is attractive for application to high throughput bottom-up proteomics. While there is a rich literature on the preparation of immobilized trypsin, there are very few studies that investi...
PubMed
Kunitz‐type trypsin inhibitor from durian (Durio zibethinus) employs a distinct loop for trypsin inhibition
Protein Sci
Kunitz‐type trypsin inhibitors are ubiquitous in plants. They have been proposed to be a part of a defense mechanism against herbivores. Trypsin inhibitors also have potential applications in the biotechnology industry, such as in mammalian cell cu...
PubMed
Dual-Mode Detection of Trypsin and Screening of Trypsin Inhibitors on the Basis of a Colorimetric/Fluorescence Sensing Probe
Anal Chem
Trypsin is a specific and reliable diagnostic biomarker of pancreatic function and pathological changes. Thus, detecting trypsin and screening trypsin inhibitors are highly important for clinical diagnosis and disease treatment. Herein, we propose a ...
PubMed
Highly stable trypsin-aggregate coatings on polymer nanofibers for repeated protein digestion
Proteomics
A stable and robust trypsin-based biocatalytic system was developed and demonstrated for proteomic applications. The system utilizes polymer nanofibers coated with trypsin aggregates for immobilized protease digestions. After covalently attaching an ...
PubMed
Leukocytes are required for the trypsin-induced increase in lung vascular permeability.
Am J Pathol
The authors examined the role of leukocytes in mediating the increase in lung vascular permeability induced by trypsin infusion in the sheep lung lymph preparation. One group of sheep was challenged with an intravenous infusion of trypsin (4.5 mg/kg/...
PubMed
Re-evaluation of Urinary Trypsin Inhibitor on Pregnancy Course in Patients with Threatened Preterm Delivery: A Single-Center Retrospective Study
Yonago Acta Med
BACKGROUND: We evaluated the necessity of urinary trypsin inhibitor for patients with threatened premature labor. METHODS: We enrolled 146 women with singleton pregnancies who were treated for threatened premature labor as inpatients. The uterine cer...
PubMed
Affinity chromatography of bovine trypsin. A rapid separation of bovine α- and β-trypsin
Biochem J
Affinity adsorbents for bovine trypsin were prepared by covalently coupling p-(p′-amino-phenoxypropoxy)benzamidine to cellulose and to agarose. Trypsin binds to both adsorbents at pH6–8 and is released at low pH values or in the presence of n-but...
PubMed
Measurement of trypsin in duodenal juice by radioimmunoassay.
Gut
Trypsin in duodenal aspirate and pure pancreatic juice samples has been measured by both radioimmunoassay and enzymatic mathods. The radioimmunossay has been shown to be specific and to detect trypsin in the presence of aprotinin (Trasylol). In duode...
PubMed
Serum immunoreactive trypsin concentration after a Lundh meal. Its value in the diagnosis of pancreatic disease.
J Clin Pathol
The changes in serum trypsin concentration have been measured in 47 subjects for up to 2 hours after a Lundh meal. In 18 healthy controls, mean fasting trypsin concentration was 285 +/- 125 ng/ml (mean +/- 2 SD). The maximum increase after the Lundh ...
PubMed
Pseudotrypsin: A Little-Known Trypsin Proteoform
Molecules
Trypsin is the protease of choice for protein sample digestion in proteomics. The most typical active forms are the single-chain β-trypsin and the two-chain α-trypsin, which is produced by a limited autolysis of β-trypsin. An additional intra-chai...
PubMed
Interaction mechanism of aloe-emodin with trypsin: molecular structure–affinity relationship and effect on biological activities
RSC Adv
The molecular mechanism of interaction between aloe-emodin (AE) and trypsin was investigated, exhibiting remarkable outcomes. To detect the interaction mechanism, the binding of AE with trypsin was examined by a multi-spectroscopy and molecular docki...
PubMed
Proteolytic Activation of the Human Epithelial Sodium Channel by Trypsin IV and Trypsin I Involves Distinct Cleavage Sites
J Biol Chem
Proteolytic activation is a unique feature of the epithelial sodium channel (ENaC). However, the underlying molecular mechanisms and the physiologically relevant proteases remain to be identified. The serine protease trypsin I can activate ENaC in vi...
PubMed
Trypsin causes platelet activation independently of known protease-activated receptors
Thromb Haemost
To identify a physiological agonist of PAR3, we used PAR4 null murine platelets, which were known to express only PAR3. In this study, we tested several proteases and found that trypsin, but not heat-inactivated trypsin, activated PAR4 null murine pl...
PubMed
Characterization of the Interactions between Minocycline Hydrochloride and Trypsin with Spectroscopic and Molecular Docking Technology
Molecules
In the current study, the interaction of minocycline hydrochloride (MC) and trypsin (TRP) was studied using fluorescence spectroscopy, synchronous fluorescence spectroscopy, three-dimensional fluorescence spectroscopy, UV-Vis spectroscopy, and molecu...